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Isolated CD4 + and CD8 + T cells were activated or not for up to 1, 3, or 6 days in a reverse time course with Dynabeads (1:1 cell-to-bead ratio, Gibco), magnetic beads similar in size to antigen-presenting cells and coupled with anti-CD3 and anti-CD28 antibodies, so that their total time in culture would be identical and to ensure that all cells would finish their indicated activation period the same day

Avocado-derived bioactive compounds, along with other antioxidants such as quercetin, fatty acids, and polyphenols, mitigate oxidative stress by modulating mitochondrial function and NADPH oxidaserelated pathways, thereby reducing excessive ROS production and oxidative damage

2.13 Western blot analysis for protein expression SH-SY5Y cells were cultured in six-well plates and subjected to the treatments as described in Section 2.7

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