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Conjugated bile acids are more efficiently secreted into bile and are important for fat digestion, cholesterol disposal and the delivery of biliary compounds into the intestine

While LPS exposure alone resulted in a 59% increase in oxidized DCF (a measure of ROS) compared to control cells, pretreatment with GHK-Cu at concentrations of 1, 5, and 10 M significantly decreased ROS production

The animal studies used both local application at the injury site and systemic administration (intraperitoneal and intragastric), with both routes showing significant effects

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Luciferase and renilla signals were measured 48 h after transfection using the Dual Luciferase Reporter Assay Kit (Promega, Madison, WI, USA) according to a protocol provided by the manufacturer