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Onishi et al., 2021

For instance, SFN inhibited the viability of about 70% of HepG2 cells after exposure for 48 h at 20 M, but no significant cytotoxicity was observed in cultured human hepatocytes when exposed to 50 M of SFN for 48 h (Zhang et al., 2020)

In specific embodiments, Ab is an anti-FRa antibodies which comprises the 6 CDRs which are 100% identical to corresponding CDRs of mirevtuximab, and the framework amino acid regions which are at least 90%, 91 %, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% identical to the corresponding framework amino acid regions of mirvetuximab, wherein said anti-FRa antibody has the following properties: (i) it binds to the folate receptor receptor a (FRa) as determined by Elisa assay (as described in the Examples) with an EC50 below 5nM, for example about 0.5nM

Anne KH Leung, FHKAM (Anaesthesiology), FJFICM 1 1 Department of Intensive Care, Queen Elizabeth Hospital, Hong Kong SAR, China 2 Cardiovascular Centre, St Paul's Hospital, Hong Kong SAR, China Corresponding author: Dr SY Au ( [email protected] ) Case presentation On 19 June 2022, a 45-year-old man was admitted to intensive care unit with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia

Apoptosis assays Unless otherwise stated, the cancer cells were stimulated with TNF- (10 ng/ml) plus cycloheximide (10 g/ml) for 6 h