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The Glu is quickly reunited with another amino acid (usually a Cys) and transported into the cell as -Glu-amino acid

The membranes were blocked with 5% BSA and incubated with primary antibodies at 4 C for 12 h, followed by secondary antibodies (HRP-conjugated Goat Anti-Mouse IgG, Proteintech, SA00001-1, 1:20000) for 1 h at room temperature

Tsochatzis E, Papatheodoridis GV, Manolakopoulos S, Tiniakos DG, Manesis EK, Archimandritis AJ

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After air-drying, the dye bound to the adherent biofilm was solubilized using 200 l of 33% ( v/v) glacial acetic acid, and absorbance was measured at 620 nm (A 620 ) using Varioskan Flash Multimode Reader (Singapore) with SkanIt Software 2.4.3