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Vitamin B12 deficiency and hyperhomocysteinemia in rural and urban Indians

Cell lysis was performed by immersing the slides in lysis buffer (2.5 M NaCl, 100 mM Na 2 EDTA, 10 mM Tris Base, 1% Triton X-100, 10% DMSO, pH 10) at 4 C for 1 h or overnight