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In the absence of large epidemiological studies, both in vivo and in vitro studies can delineate the molecular initiating event and subsequent key pathways that lead to potential adverse outcomes ( Experimental in vivo models of MPs exposure during pregnancy have demonstrated increased cytotoxicity, oxidative stress, inflammation, and cognitive and neurological changes in the offspring ( In vitro , studies using placental cell lines, such as HTR-8/SVneo, Bewo and Swan 71 cells have demonstrated that PS MPs can be internalized and change certain cell functions, which in turn, would alter the placenta physiology ( 2 Materials and methods 2.1 Sample collection Term placentas were obtained from a cohort of 40 anonymous healthy pregnant women (average age of 25.9 5.2 years old), carefully matched for gestational delivery week (37.8 2.6 weeks), who provided consent following uncomplicated pregnancies and underwent caesarean section deliveries

doi: 10.1128/MCB.02415-06 59 LeePJJiangBHChinBYIyerNVAlamJSemenzaGLet al

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