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anti-SUMO2/3 [8A2] 1:500 Sigma) diluted in blocking buffer (1.5% Triton X-100, 10% cosmic calf serum in 1X PBS) overnight at 4C

doi: 10.3892/etm.2018.5779 127 PerelliFMatteiAScambiaGCavaliereAF

When compared to uninfected cells in terms of single metabolites, latently infected cells showed significantly decreased levels of the initial glycolytic metabolite, i.e., glucose6phosphate, as well as of the metabolite produced by PFK, i.e., fructose 1,6 bisphosphate (Figs 2C and EV3C)

10.1016/j.actbio.2021.11.041 122

The fate of the liposomes in the cell cultures was assessed by fluorescence microscopy using a NIKON TE2000-E inverted microscope, triple band filter for Rhodamine, GFP and DAPI at 2Ox or 6Ox magnification