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This is important so that change in rare symptoms, such as seizures, are only evaluated in participants with those symptoms

Bioinformatics 36 , i813i821 (2020)

Brain blocks were rinsed three times with 0.1 M phosphate buffer solution and cryoprotected in 10, 20 and 30% (w/v) sucrose in phosphate buffer solution sequentially, until they sank

The six NIST serum samples were thoroughly vortexed

Discussion The studies reported here provide the first detailed survey and investigation of the ultrastructural and histological state of the brain and of regions taken from the whole brain after vitrification and rewarming and after the introduction and partial removal of vitrifiable concentrations of cryoprotective agents