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Variants S463D and S463K of EcGGT showed no detectable activities with considerable maturational defects (Hsu et al., 2009), while S463T mutant displayed 40% loss in activity without affecting autoprocessing, indicating that presence of hydroxyl group is required for autoprocessing and addition of charged residues at this position is detrimental for both processing and activity

Thus, even though a plausible pathogenesis, this mechanism has not been replicable depending on the type of cell lines and in-vivo v/s in-vitro studies