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For confocal microscopy, BMDM were treated with or without 250 M palmitate for 24 hours and incubated with 200 nM mitotracker green, 200 nM mitotracker deep Red (Excitation/emission: 644/665, in the Cy3 channel), and 5 M of mitosox red (PE), and counterstained with 300 nM DAPI (Invitrogen, #D1306) for 30 minutes in PBS

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Ta obserwacja skonia badaczy do postawienia hipotezy, e suplementacja GHK-Cu zarwno miejscowa, jak i iniekcyjna mogaby czciowo przywrci procesy naprawcze i regeneracyjne osabione przez starzenie