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Similarly, the restoration of neurotrophic support, whether through exogenous administration, gene therapy, or immune-mediated modulation, illustrates a growing sophistication in therapeutic design, although questions regarding long-term efficacy, immune tolerance, and BBB penetration remain unresolved

To construct a random mutagenesis library based on the HpaB mutant 23F9, Error-prone PCR was performed using plasmid pFA- hpaB 23F9 C as template with primers hpaB -ErrorProne-for and hpaB -ErrorProne-rev

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