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Additionally, chronic conditions such as HIV and tuberculosis have been implicated in B12 deficiency due to increased metabolic demand and altered absorption mechanisms ( The diagnosis of vitamin B12 deficiency typically relies on low total B12 levels and elevated concentrations of homocysteine (Hcy), as well as levels of transcobalamin-bound B12 (holoTC) and methylmalonic acid (MMA), which serve as functional indicators of cobalamin insufficiency ( Vitamin B12 requirements fluctuate throughout the life course, influenced by metabolic demand and physiological changes

532 Apart from their roles above in redox balance and immune response, mitochondria also involve in proteostasis and other cellular functions that intensively interact with ER and nucleus

The 30 mutants were induced to express the protein Ch PPK

More interestingly, they also showed that many variations around this model exist depending on the initial conditions, revealing the complexity of OR regulation now translated to the concept of biased agonism