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A second nested-PCR enrichment step was performed for 11 cycles using the same cycling parameters as before, using the entire bead-purified reaction product from the first step as a template with primers YL009 (CAAGCAGAAGACGGCATACGAG) and JBD-SS-273:280 (AATGATACGGCGACCACCGAGATCTACAC TATAGCCT ACACTCTTTCCCTACACGACGCTCTTCCGATCT NNNNNNN GCAGGGATGTCCACGAGGTCTCT) where N 8 barcodes correspond to reverse-complemented Illumina TruSeq HT D501-508 indexes

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7 ) implying that such changes may in fact be occurring in the intact hippocampus

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6/16/8 2019), the protocol of the Czech post-MONICA study by the Ethics Committee of the Institute for Clinical and Experimental Medicine and Thomayer Hospital, Prague, Czech Republic (No