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Total RNA was treated with DNase I (Sigma-Aldrich), quantified, and reverse transcribed into cDNA using the ReverTra Ace- First Strand cDNA Synthesis Kit (TOYOBO, Osaka, Japan)

Kalpana Sundar, DO Learn More Safety & Treatment Considerations Peptide therapy should always be discussed within a physician-led context

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Then, all mice were euthanized, and knee joints were harvested for subsequent analysis