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Many of the physiological problems that astronauts experience in space, such as motion sickness and otolith dysfunction as well as cardiovascular, bone, and muscle deterioration, are caused by the absence of normal terrestrial gravity (1 g) and the ensuing lack of mechanical stimulation of cells and tissues

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Portions upstream (443 bp) and downstream (338 bp) of the GDH2 gene were amplified and fused with the 5269 bp SAT1 fragment to form a disruption cassette (~6 kb) for GDH2 gene deletion (Supplementary Fig