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I didnt see if it was one or more cells, but from experience going down to 3.1 or below makes it almost impossible to balance multiple cells ever again

S.VaresiA.FlorisV.SavioliG.MantovaniE.Lpez-CarrascoP.et al (2021)

Histopathological findings in testicular, epididymal, semen vesicle glandular and prostatic structures in control and treated rats The histological results of the various organs under investigation, such as testes, prostate glands, seminal vesicles and epididymis, were depicted in Figs

Because these histidines are expected to be required for 9 desaturase activity we expressed this allele in mutant yeast that lack 9 desaturase activity ( ole1 mutants)

doi: 10.1073/pnas.2212476120 125 Azarcoya-BarreraJWollinBVeida-SilvaHMakarowskiAGorukSFieldCJet al