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The cells were then fixed on ice with Fixation Buffer (#420801, Biolegend, USA) for 20 min, and stained with cytokine antibodies (listed in Supplementary Table 1) in permeabilization buffer (#421002, Biolegend, USA)

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Effects and potential mechanisms of pioglitazone on lipid metabolism in obese diabetic KKAy mice

Discovery of a SUCNR1 antagonist for potential treatment of diabetic nephropathy: in silico and in vitro studies