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Our observation that intracellular NA concentrations did not increase following NNMT inhibition extends previous studies that showed NA concentrations were largely unaffected following NNMT knockdown with either adenovirus[39] or anti-sense oligonucleotides (ASOs).[12] It is possible that significant accumulation of NA in adipocytes (and other tissues) is prevented by rapid conversion of NA to NMN and subsequent NAD + synthesis or transport to the extracellular environment.[27] Importantly, NNMT inhibition potentiated the absolute intracellular concentrations of NAD + and SAM, without altering the levels of NA and SAH in the adipocytes

However, the parenteral use of vitamin B12 has proven itself not only in pernicious anemia, the classic B12 deficiency disease, but also in polyneuropathies and mental disorders

Dose optimization, dietary adjustments, and exercise modifications typically produce better results than adding unvalidated combination protocols

Note that this supplementation does not result in 100% Eu animals similar to supplementation with lower concentrations of vitamin B12 (Fig