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Description

Taken together, these data indicate that isoxazole 1 displays high selectivity for Cn Acs1, the enzyme upon which the chemical-genetic screen was designed

For GST pull-down assay, the proteins were purified and GST or GST-TMED10 was incubated with Glutathione agarose (GE, 17-0756-05) (which was blocked by 10% FBS) in IP buffer used for Co-IP, and rotated at 4 C for 1 h

Dimmock D, Maranda B, Dionisi-Vici C, Wang J, Kleppe S, Fiermonte G, Bai R, Hainline B, Hamosh A, OBrien WE, Scaglia F, Wong LJ (2009) Citrin deficiency, a perplexing global disorder