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Following centrifugation, the resulting pellet was lysed in the specified lysis buffer supplemented with a protease inhibitor cocktail

[DOI] [PubMed] [Google Scholar] 90.Holbrook A.M

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Introduction Diabetes mellitus, affecting 29.1 million people in the United States ( Oxidative stress, which occurs when there is an imbalance between generation and removal of reactive oxygen species (ROS), may contribute to -cell damage during either T1D or T2D (5 Types of ROS and Relevance to -Cell Damage A critical feature of the -cell is its ability to link changes in metabolic flux, brought on by glucose metabolism, to changes in membrane excitability and subsequent alterations in intracellular Ca 2+ concentration ([Ca 2+ ] i ) and insulin secretion ( 2 on supply of the sugar substrate ( Here, we will focus on three types of ROS: superoxide (O 2 - ), hydrogen peroxide (H 2 O 2 ), and peroxynitrite (ONOO - )