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Graft-versus-host disease is locally maintained in target tissues by resident progenitor-like T cells

This may be especially true if you drink dark liquors, like bourbon and brandy

2021;13(7) doi: 10.3390/pharmaceutics13071003

Samulak JJ, Sawicka AK, Samborowska E, Olek RA

Gut microbiota inoculum preparation from human feces for ex vivo study One gram of raw fecal sample was mixed with 10 mL of PBS supplement with 0.05% -cysteine in a tube containing glass beads, homogenized the mixture by vortexing for 1 min in an anaerobic chamber (Whitley DG250 Workstation, Don Whitley Scientific Limited, UK), filtered the homogenized fecal liquid through a Falcon 100 m cell strainer, and subsequently used the fecal filtrate for the study