nadph and glutathione levels gliomas Insight into the metabolism of by MRI MRS | Biophysical Reviews Cysteine induces mitochondrial reductive stress

To determine the total glutathione (GSH + 2GSSG) concentration, the following stock solutions were freshly prepared in KPE (0.1 M potassium phosphate, 5 mM EDTA, pH 7.5): 5,5-dithio- bis -2-nitrobenzoic acid (DNTB) at 0.6 mg/mL, NADPH at 0.6 mg/mL and glutathione reductase at 3 units/mL

Lytrivi M, Castell AL, Poitout V, Cnop M

Two different chromatographic methods were used

Detection of lipid peroxidation products The Click-iT Lipid Peroxidation Imaging kit (ThermoFisher Scientific) with Alexa Fluor 488 azide was used to detect macromolecules modified with breakdown products from peroxidized lipids according to the recommendations of the manufacturer

Jin Y, Li F, Li Z, Ikezu TC, OLeary J, Selvaraj M, et al