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GST tagged fusion proteins bind to glutathione agarose and, after washing, are eluted with elution buffer with excess glutathione Reducing agents are common additions to protein purification buffers because they help keep many proteins from forming unnatural disulfide bonds and aggregating

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These component values are mathematical breakdowns of the blend, not dose recommendations

(ii) the -ketoglutarate dehydrogenase complex that catalyzes the conversion of -ketoglutarate to succinyl CoA, another important intermediate of the citric acid cycle