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Extracted peptides were subjected to nanoLC-ESI-MS/MS mass spectrometry (LTQ Orbitrap Discovery or Orbitrap Q Exactive Plus, Thermo Fisher Scientific) using an EasyNano LC system equipped with an ODS column (particle diameter 3 m, 0.075 mm 125 mm, Nikkyo Technos, Tokyo, Japan)

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For example, purification of virus-host protein complexes from infected plants coupled to mass spectrometry identified a GST co-purifying with Rice yellow mottle virus (RYMV) in a partially resistant rice cultivar but not in a susceptible one (Brizard et al., 2006)