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3e, in both A375 and B16F10 cells, only DeTYR-3 formed from the TYR-catalyzed VH032-Azi3 + Alk-TIn + SA group could successfully form a ternary complex between TYR, DeTYR-3, and VHL, as evidenced by the detected TYR in the final precipitated protein samples pulled down by anti-VHL antibodies in VH032-Azi3 + Alk-TIn + SA group rather than DMSO and epi-VH032-Azi3 + Alk-TIn + SA control groups

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9D, the dependency of long-chain FAs oxidation was obviously higher in SIRT3OE adipocytes than that in the vector cells, but not pyruvate or glutamine, suggesting that SIRT3OE cells can switch their metabolic phenotype to be more reliant on FAO

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Pathogen-avoidance assays Pathogen-avoidance assays were performed according to a previously described method 32 , with minor modifications