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If long-term storage of a reconstituted solution is required, researchers typically aliquot the solution into single-use vials, snap-freeze at -80 C, and thaw only once before use

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The NHS advises against very low calorie diets (VLCDs) of fewer than 800 kcal per day unless they are medically supervised

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Research in animal models has shown significant reductions in body weight and adipose tissue, with studies reporting up to 50% less weight gain in obese Zucker rats after 19 days of administration