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The following day, cells were washed once with each buffer: low salt (0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH 8.0, 150 mM NaCl), high salt (0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH 8.0, 5,000 mM NaCl), LiCl (0.25 M LiCl, 1% NP-40, 1% sodium deoxycholate, 1 mM EDTA, 10 mM Tris-HCl pH 8.0) and eluted in 1% SDS, 100 mM NaHCO 3 buffer

The main byproducts are shown in Table 7

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Analysis of peptides presented on MHC molecules in human macrophages (differentiated THP-1 cells) infected with either BCG or MTB led to the identification of 64 BCG-derived and 80 MTB -derived antigens