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Briefly, tissue was dissected, incubated in a solution of 0.05% Trypsin/EDTA, washed with Hanks balanced salt solution (HBSS, Thermo Fisher Scientific), dissociated by trituration and distributed into poly-L-lysine coated 12-well plates (Costar, Cambridge, MA) with and without inserted glass coverslips containing DMEM/F12 medium with 10% fetal bovine serum

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Cell Signal 24:981990

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