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Once -arrestin is bound to a GPCR, it undergoes a conformational change allowing it to serve as a scaffolding protein for an adaptor complex termed AP-2, which in turn recruits another protein called clathrin

At the respective analysis point, the medium was removed, washed with 200 l PBS and replenished with 200 l fresh medium per well

The temperature was in the mid-nineties

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Magoffin DA, Weitsman SR: Insulin-like growth factor-I regulation of luteinizing hormone (LH) receptor messenger ribonucleic acid expression and LH-stimulated signal transduction in rat ovarian theca-interstitial cells