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SDS-PAGE Protein samples (2 g) were analyzed on 12% Bis-Tris Bolt SDS-PAGE gels (ThermoFisher) with Bolt MES SDS running buffer (ThermoFisher)

Currently, about 54 copper-binding proteins have been discovered, with approximately half being enzymes, 12 being copper transporters, and one being a transcription factor antioxidant 1 copper chaperone (ATOX1) (23)

However, oral zinc may cause gastrointestinal discomfort, nausea, or poor absorptionespecially during illness

Advancing research has shown that their discovery is driving a paradigm shift in the study of NDDsfrom the traditional emphasis on suppressing inflammation to the active promotion of inflammation resolution. This conceptual transition not only deepens our understanding of disease pathogenesis but also creates new opportunities for clinical interventions

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