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This involves the comparison of mass fragmentation spectra (MS/MS spectra) experimentally acquired in the study through data dependent acquisition (DDA) or data independent acquisition (DIA) against the spectral and retention time (RT) databases of known or predicted metabolites

Peptides as a class are not banned

Free radicals are unstable molecules generated by environmental stressors like UV radiation, pollution, and smoking

Bile acids are also conjugated by a similar sequence of reactions involving a microsomal cholylCoA synthetase and a cytosolic enzyme bile acidCoA: amino acid acyltransferase (Falany et al., 1994)

The skin on the face is thinner than most body areas, making these changes more visible