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Description

Total RNA isolation was carried out using an Omega RNA-Solv Reagent kit (BIO-TEK, LOT: R6830118886-33, USA) following the manufacturers protocol

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739 In the MCAO/R model of rats, the expression of SLC7A11 is downregulated, accompanied by inhibition of GSH and GPX4, suggesting that the deficiency of xCT in cerebral I/R injury may be the main cause of lipid peroxidation and ferroptosis

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The levels of MDA, GSH, and SOD were measured according to the instructions provided with the respective assay kits (Jiancheng Bioengineering Institute, Nanjing, China)

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