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As the implanted tumour cells lack p53 expression (ID8 Trp53 / ), and p21 is a p53-dependent gene, we wanted to confirm that (a subset of) the p21-positive cells were tumour derived

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[2] The classic experimental tool that exposed how central this synthetic pathway is has been the inhibitor buthionine sulfoximine, which blocks glutamate-cysteine ligase and lets researchers deliberately deplete cellular glutathione to observe the consequences

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