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To Eliminate non-specific adsorbents caused by hydrophobic adsorption: Wash with 3-4 column bed volumes of 70% ethanol or 2 column bed volumes of 1% Triton X-100, followed immediately by 5 column bed volumes of PBS, pH 7.4.

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Fluorescence intensity was measured using flow cytometry (Becton Dickinson Accuri C6) with excitation at 488 nm and emission at 530 nm

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