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Micro RNAs (miRNAs) miR-141, miR-432-3p, miR-200a, have also shown to modulate the activities of KEAP1 and NRF2 in ovarian carcinoma cell lines, breast cancer, esophageal squamous cell carcinoma (ESCC), endometrial cancer tissues, miR-7, directly targeted KEAP1 mRNA in neuroblastoma SH-SY5Y cells, where it activated NRF2-dependent transcription of the antioxidant genes HMOX1 and GCLM [49]

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Cultures were imaged with the Opera Phenix Plus High-Content Screening System (PerkinElmer) for 4 h at 37 C with 5% CO 2