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[DOI] [PubMed] [Google Scholar] 271.Ko RK, Kim GO, Hyun CG, et al

However, the form you choose is only part of the equation

The cells were then washed with PBS buffer, lysed with lysate buffer [100 l containing 50 mM PBS, 0.1 mM phenylmethanesulfonyl fluoride (5 l) and Triton X-100 (5 l)], and frozen at 80C for 30 min