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The quantitative reaction was performed on a 7500 Fast Real-Time PCR System (Applied Biosystems) using GoTaq qPCR Master Mix kit (Promega)

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Expression of -2-microtubilin (B2M) was used for normalization of CT data according to the CT method 63

Normal IGF-1 ranges 182780 ng/mL for people between 16 and 24 years 114492 ng/mL for people between 25 and 39 years 90-360 ng/mL for people between 40 and 54 years 71-290 ng/mL for people older than 54 years When it's too high This might be a sign of acromegaly, especially if the growth hormone levels are also high

Pratic D., Evidence of Oxidative Stress in Alzheimer's Disease Brain and Antioxidant Therapy: Lights and Shadows, Annals of the New York Academy of Sciences 1147 (2008): 7078