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Description

The fluorescence was analyzed by flow cytometry (Attune NxT, ThermoFisher Scientific)

Subsequent purification included size exclusion chromatography using a Superdex 200 column (GE Healthcare) and further purification steps with glutathione Sepharose 4B and Ni Sepharose resins to remove residual GST and TEV protease

The remaining L1FnIII-2 interaction maintains the unliganded half of the IGF1R dimer in the apo state, resulting in the asymmetric architecture

& Cheng, T

While this limitation exists, this shouldnt be taken as a flaw in the experimental design, since this method is suitable to support FBBD campaigns 100