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8,9 Table 1 Risk Factors for Vitamin B12 Deficiency A multicenter randomized controlled trial of 390 patients with diabetes mellitus showed that those taking 850 mg of metformin three times per day had an increased risk of vitamin B 12 deficiency (number needed to harm = 14 per 4.3 years) and low vitamin B 12 levels (number needed to harm = 9 per 4.3 years) vs

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Deletion of the gene encoding the vacuolar protease Pep4, a key protease required for the activation of most vacuolar enzymes, also abolished autophagy flux, indicating that the observed cleavage of free GFP was generated from vacuolar hydrolysis (Fig

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