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Results Non-stimulated as well as histamine-, IL-4- and IL-13- stimulated mast cells from AD patients show higher mRNA expression levels of 5-lipoxygenase (5-LO) and 5-LO activating protein (FLAP) compared to cells from HVs The eicosanoid metabolizing enzymes 5-LO and FLAP are acting jointly to convert AA to the unstable epoxide LTA4, which is the substrate for the leukotriene biosynthetic enzymes LTC4S and LTA4H [19]