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After 3 h of treatment, the most impacted pathways, revealed by 40 significantly changed metabolites, included amino acid metabolism (alanine, aspartate, and glutamate), the pentose phosphate pathway, the TCA cycle, purine metabolism, and pyrimidine metabolism (Figure 4A)

The original 1977 Monnier study showed that dialysate from sleeping rabbit brains (containing DSIP) could induce delta-wave sleep when infused into waking rabbits

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The compound's mechanism of action operates through HGF mimetic activity, binding to c-Met receptors expressed on neurons and triggering intracellular signaling cascades that promote synaptogenesis, neurite outgrowth, and dendritic spine formation