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In many species, including prokaryotes, plants, yeast, and metazoans, monothiol glutaredoxins may be found in complex with BolA-like proteins, with distinct complexes formed in the cytosol/nucleus, mitochondria, and plastids

As a regulatory molecule which appeared very early in evolution, GHK may hold the key to health and wellness through multiple mechanisms, which include regulating copper, activating pathways linked to repair and regeneration as well as acting directly on DNA, modulating gene expression

Taniguchi et al

After washing, the membranes were incubated with appropriate secondary antibodies for 1 h at room temperature