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An animal is considered to be in the open area when all four paws are in an open quadrant and in the closed area only when all four paws have crossed from the open to the closed boundary

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For example, the inhibition of histone deacetylases or demethylases interferes with the chromatin modifications that maintain DTP cells in a drug-tolerant state, such as the retention of closed or repressed chromatin at pro-apoptotic loci, thereby restoring susceptibility to drug-induced apoptosis (55)