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Cells were maintained under standard conditions: cultured in DMEM supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin, and incubated at 37 C in a humidified environment with 5% CO

Umbilical Cord MSCs and Bone Regeneration Umbilical cord MSCs differentiate osteogenically when GHK combines with alginate hydrogel, showing 10-fold Runx2 mRNA increases before chemical induction through ILK (integrin-linked kinase) activation

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