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The cultivation of CTCs in vitro encounters two primary challenges

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Glutamine metabolism NSCLC cells demonstrate high dependence on glutamine for survival and proliferation, a phenomenon termed glutamine addiction. As illustrated in Table 1, glutamine serves as a critical source of carbon and nitrogen, feeding into the tricarboxylic acid (TCA) cycle through glutaminolysis and supporting production of nucleotides, amino acids, and the antioxidant glutathione

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In contrast, pre-immune sera controls failed to label the nozzles (Fig