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Biochem J 29:16021611 Hino T, Harada M, Maekawa H (1985) Production of high-glutathione-containing yeast cells

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Briefly, 3 mL of 0.15 M NaOH and 1 mL of 3% formaldehyde were added to 2 mL of the cell disruption supernatant and mixed well

Authors performed an impressive number of experiments, aimed to demonstrate that RT preferentially reacts with GSH under physiological conditions