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328(Pt 1), 199203

Dissociation between CSD-evoked metabolic perturbations and meningeal afferent activation and sensitization: implications for mechanisms of migraine headache onset

They were given broccoli sprout extract (BSE) to take daily before surgery, with 200 mol of isothiocyanate (ITC) being the active ingredient

The medium was composed of AdDMEM/F12 (Invitrogen) supplemented with 1% HEPES, 1% GlutaMAX (ThermoFisher), 1% penicillin/streptomycin (ThermoFisher), 1 B27 without retinoic acid (Gibco), 1.25 mM N -acetylcysteine (Sigma), 10 nM gastrin (Sigma) and the following growth factors: 50 ng ml 1 hEGF (Peprotech), 15% RSPO1 conditioned medium (home-made), 100 ng ml 1 FGF10 (Peprotech), 100 ng ml 1 FGF7 (Peprotech), 50 ng ml 1 HGF (Peprotech), 10 mM nicotinamide (Sigma, for EM1 medium only), 2 M A83-01 (Tocris), 3 M CHIR99021 (Tocris), 10 M Y-27632 (Tocris), 0.5 nM Wnt surrogate Fc fusion protein as in ref