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doi: 10.1038/nrc1093

When the body does not produce enough testosteronea condition known as hypogonadismpatients may experience symptoms such as fatigue, decreased libido, difficulty concentrating, depression, and loss of muscle mass

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After overnight centrifugation, the band containing the nozzles was collected using a gradient fractionator (Labconco Auto Densi-Flow), dialyzed against Tris buffer, and the nozzles were either collected by centrifugation (1 h at 366,000 g ) or further purified using 30 ml gradients of 10-40% sucrose (wt/wt)

Water solubility Water solubility is an important parameter, which gives information on the polarity of the molecule and its capacity to be extracted by an aqueous solvent or to form hydrogen bond interactions