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Cytochrome c- and caspase-dependent apoptosis was subsequently observed, yet incubation with the antioxidants glutathione, -tocopherol and N-acetylcysteine, either alone or in combination, was unable to prevent cellular death [38]

In particular, 1 mL of a 1 mM solution of vicine prepared in 50 mM phosphate buffer at pH 5, preliminarily flushed with nitrogen for 10 min, was subjected to enzymatic hydrolysis by adding 5 mg of almond -glucosidase (Sigma) and incubating at room temperature, in accordance with the experimental conditions reported by Pedersen, Musci and Rotilio 33

Xenobiotica 18:144955 Dickie AP, Wilson CE, Schreiter K, Wehr R, Wilson EM, Bial J, Scheer D, Wilson ID, Riley RJ (2017) The pharmacokinetics and metabolism of lumiracoxib in chimeric humanized and murinized FRG mice