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While DIA typically yields increased peptide identifications compared to DDA, it often produces a lower proportion of confidently localised sites due to the increased spectral complexity, a challenge that is further exacerbated in low-input workflows, where the intensity of diagnostic fragment ions is reduced (Muneer et al., 2024, Kitata et al., 2021)

Bukata, M.D., and Freda B

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For example, research utilizing microglial extracellular vesicles (EVs) from cryopreserved AD brain tissue analyzed lipidomic, transcriptomic, and proteomic datasets to illustrate how microglial activity shifts in late-stage AD, impacting the clearance of tau-laden neuronal debris (266)

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